› 2qfn

X-ray diffraction
1.62Å resolution

X-ray Crystal Structure Analysis of the Binding Site in the Ferric and Oxyferrous Forms of the Recombinant Heme Dehaloperoxidase Cloned from Amphitrite ornata

Released:
DOI: 10.2210/pdb2qfn/pdb
PDB entry 2qfn coloured by chain, front view.
PDB entry 2qfn coloured by chain, side view.
PDB entry 2qfn coloured by chain, top view.
Source organism: Amphitrite ornata
Primary publication:
X-ray crystal structural analysis of the binding site in the ferric and oxyferrous forms of the recombinant heme dehaloperoxidase cloned from Amphitrite ornata.
de Serrano V, Chen Z, Davis MF, Franzen S
Acta Crystallogr D Biol Crystallogr 63 1094-101 (2007)
PMID: 17881827

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Globin domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 138 amino acids
Theoretical weight: 15.66 KDa
Source organism: Amphitrite ornata
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NAV8 (Residues: 1-138; Coverage: 100%)
Sequence domains: Globin
Structure domains: Globins

Ligands and Environments

4 bound ligands:
Ligand NH4 2 x NH4
Ligand SO4 3 x SO4
Ligand HEM 2 x HEM
Ligand OXY 2 x OXY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 57.938Å b: 67.091Å c: 68.402Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.17 0.224
Expression system: Escherichia coli

Quick links

Citations

11 citation in other articles

Internal binding of halogenated phenols in dehaloperoxidase-hemoglobin inhibits peroxidase function.
Thompson et al. (2010)
10 more

1 mention without citation

A model for the flexibility of the distal histidine in dehaloperoxidase-hemoglobin A based on X-ray crystal structures of the carbon monoxide adduct.
Zhao et al. (2014)