› 2qfc

X-ray diffraction
2.6Å resolution

Crystal Structure of Bacillus thuringiensis PlcR complexed with PapR

Released:
DOI: 10.2210/pdb2qfc/pdb
PDB entry 2qfc coloured by chain, front view.
PDB entry 2qfc coloured by chain, side view.
PDB entry 2qfc coloured by chain, top view.
Source organism: Bacillus thuringiensis serovar israelensis ATCC 35646
Primary publication:
Structure of PlcR: Insights into virulence regulation and evolution of quorum sensing in Gram-positive bacteria.
Declerck N, Bouillaut L, Chaix D, Rugani N, Slamti L, Hoh F, Lereclus D, Arold ST
Proc Natl Acad Sci U S A 104 18490-5 (2007)
PMID: 17998541

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-237222 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
HTH cro/C1-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 293 amino acids
Theoretical weight: 35.06 KDa
Source organism: Bacillus thuringiensis serovar israelensis ATCC 35646
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q45782 (Residues: 1-285; Coverage: 100%)
Gene names: A3L20_28550, B4918_30015, CAB88_27845, CN398_18815, COK99_05395, CON71_19630, plcR
Sequence domains:
Structure domains: Tetratricopeptide repeat domain
C-terminus pentapeptide from PapR protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 5 amino acids
Theoretical weight: 652 Da
Source organism: Bacillus thuringiensis serovar israelensis ATCC 35646
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand PEG 4 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P61
Unit cell:
a: 85.68Å b: 85.68Å c: 189.87Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.224 0.304
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

21 review citations

From soil to gut: Bacillus cereus and its food poisoning toxins.
Stenfors Arnesen et al. (2008)
20 more

10 mentions without citation

Structural Mechanisms of Peptide Recognition and Allosteric Modulation of Gene Regulation by the RRNPP Family of Quorum-Sensing Regulators.
Do et al. (2016)
9 more