PDBe 2qcs

X-ray diffraction
2.2Å resolution

A complex structure between the Catalytic and Regulatory subunit of Protein Kinase A that represents the inhibited state


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 40.74 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
  • Canonical: P05132 (Residues: 2-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:
cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed Chain: B
Molecule details ›
Chain: B
Length: 291 amino acids
Theoretical weight: 32.72 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21
  • Canonical: P00514 (Residues: 91-380; Coverage: 76%)
Gene name: PRKAR1A
Sequence domains: Cyclic nucleotide-binding domain
Structure domains: Jelly Rolls

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P3221
Unit cell:
a: 125.809Å b: 125.809Å c: 140.941Å
α: 90° β: 90° γ: 120°
R R work R free
0.194 0.192 0.225
Expression system: Escherichia coli BL21