› 2qcb

X-ray diffraction
1.65Å resolution

T7-tagged full-length streptavidin complexed with ruthenium ligand

Released:
DOI: 10.2210/pdb2qcb/pdb
PDB entry 2qcb coloured by chain, front view.
PDB entry 2qcb coloured by chain, side view.
PDB entry 2qcb coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
X-ray structure and designed evolution of an artificial transfer hydrogenase.
Creus M, Pordea A, Rossel T, Sardo A, Letondor C, Ivanova A, Letrong I, Stenkamp RE, Ward TR
Angew Chem Int Ed Engl 47 1400-4 (2008)
PMID: 18176932

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 16.61 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 38-183; Coverage: 92%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

1 bound ligand:
Ligand KYS 1 x KYS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: I4122
Unit cell:
a: 57.651Å b: 57.651Å c: 183.823Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.168 0.187
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Optimizing non-natural protein function with directed evolution.
Brustad et al. (2011)
9 more

6 mentions without citation

Design of functional metalloproteins.
Lu et al. (2009)
5 more