› 2q6o

X-ray diffraction
2Å resolution

SalL-Y70T with SAM and Cl

Released:
DOI: 10.2210/pdb2q6o/pdb
PDB entry 2q6o coloured by chain, front view.
PDB entry 2q6o coloured by chain, side view.
PDB entry 2q6o coloured by chain, top view.
Source organism: Salinispora tropica CNB-440
Primary publication:
Discovery and characterization of a marine bacterial SAM-dependent chlorinase.
Eustáquio AS, Pojer F, Noel JP, Moore BS
Nat Chem Biol 4 69-74 (2008)
PMID: 18059261

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + chloride = 5-deoxy-5-chloroadenosine + L-methionine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107474 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosyl-chloride synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 283 amino acids
Theoretical weight: 30.11 KDa
Source organism: Salinispora tropica CNB-440
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A4X3Q0 (Residues: 1-283; Coverage: 100%)
Gene names: Strop_1026, salL
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand SAM 2 x SAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P3
Unit cell:
a: 111.53Å b: 111.53Å c: 47.61Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.269 0.266 0.313
Expression system: Escherichia coli BL21

Quick links

Citations

41 review citations

Genomic basis for natural product biosynthetic diversity in the actinomycetes.
Nett et al. (2009)
40 more

6 mentions without citation

Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse.
Agarwal et al. (2017)
5 more