PDBe 2q3c

X-ray diffraction
2.1Å resolution

2.1 A Resolution Crystal Structure of O-Acetylserine Sulfhydrylase (OASS) Holoenzyme From MYCOBACTERIUM TUBERCULOSIS in Complex with the Inhibitory Peptide DFSI

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
O-acetylserine sulfhydrylase Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 33.3 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WP55 (Residues: 1-310; Coverage: 100%)
Gene names: MTCY98.03, Rv2334, cysK, cysK1
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold
DFSI inhibitory peptide Chain: B
Molecule details ›
Chain: B
Length: 4 amino acids
Theoretical weight: 481 Da
Source organism: Mycobacterium tuberculosis
Expression system: Not provided

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P41212
Unit cell:
a: 72.458Å b: 72.458Å c: 178.936Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.207
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided