PDBe 2q0o

X-ray diffraction
2Å resolution

Crystal structure of an anti-activation complex in bacterial quorum sensing

Released:
Source organism: Sinorhizobium fredii NGR234
Primary publication:
Structural basis for antiactivation in bacterial quorum sensing.
Proc. Natl. Acad. Sci. U.S.A. 104 16474-9 (2007)
PMID: 17921255

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Probable transcriptional activator protein TraR Chains: A, B
Molecule details ›
Chains: A, B
Length: 236 amino acids
Theoretical weight: 26.32 KDa
Source organism: Sinorhizobium fredii NGR234
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P55407 (Residues: 1-236; Coverage: 100%)
Gene names: NGR_a04090, traR, y4dH
Sequence domains:
Structure domains:
Probable transcriptional repressor TraM Chains: C, D
Molecule details ›
Chains: C, D
Length: 107 amino acids
Theoretical weight: 12.22 KDa
Source organism: Sinorhizobium fredii NGR234
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: NEW P55408 (Residues: 1-107; Coverage: 100%)
Gene names: NGR_a04080, traM, y4dI
Sequence domains: Prokaryotic Transcriptional repressor TraM
Structure domains: HR1 repeat

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P1
Unit cell:
a: 56.9Å b: 62.48Å c: 65.5Å
α: 94.87° β: 110.45° γ: 99.17°
R-values:
R R work R free
0.235 0.235 0.276
Expression system: Escherichia coli BL21(DE3)