PDBe 2q0n

X-ray diffraction
1.75Å resolution

Structure of human p21 activating kinase 4 (PAK4) in complex with a consensus peptide

Released:
Entry authors: Filippakopoulos P, Eswaran J, Turnbull A, Papagrigoriou E, Pike AW, Von Delft F, Sundstrom M, Edwards A, Arrowsmith CH, Weigelt J, Knapp S, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Synthetic peptide Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.56 KDa
Source organism: Synthetic construct
Expression system: Not provided
Serine/threonine-protein kinase PAK 4 Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 34.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O96013 (Residues: 291-591; Coverage: 51%)
Gene names: KIAA1142, PAK4
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P43212
Unit cell:
a: 145.678Å b: 145.678Å c: 39.577Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.228
Expression systems:
  • Not provided
  • Escherichia coli