PDBe 2pz8

X-ray diffraction
2Å resolution

NAD+ Synthetase from Bacillus anthracis with AMP-CPP and Mg2+

Released:

Function and Biology Details

Reaction catalysed:
ATP + deamido-NAD(+) + NH(3) = AMP + diphosphate + NAD(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NH(3)-dependent NAD(+) synthetase Chains: A, B
Molecule details ›
Chains: A, B
Length: 284 amino acids
Theoretical weight: 31.53 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q81RP3 (Residues: 1-272; Coverage: 100%)
Gene names: BAS1855, BA_1998, GBAA_1998, nadE
Sequence domains: NAD synthase
Structure domains: HUPs

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21212
Unit cell:
a: 107.183Å b: 96.507Å c: 68.633Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.202 0.229
Expression system: Escherichia coli