› 2pqj

X-ray diffraction
2.8Å resolution

Crystal structure of active ribosome inactivating protein from maize (b-32), complex with adenine

Released:
DOI: 10.2210/pdb2pqj/pdb
PDB entry 2pqj coloured by chain, front view.
PDB entry 2pqj coloured by chain, side view.
PDB entry 2pqj coloured by chain, top view.
Source organism: Zea mays
Primary publication:
Structure-function study of maize ribosome-inactivating protein: implications for the internal inactivation region and the sole glutamate in the active site.
Mak AN, Wong YT, An YJ, Cha SS, Sze KH, Au SW, Wong KB, Shaw PC
Nucleic Acids Res 35 6259-67 (2007)
PMID: 17855394

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150608 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosome-inactivating protein 3 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 243 amino acids
Theoretical weight: 27.31 KDa
Source organism: Zea mays
Expression system: Escherichia coli
UniProt:
  • Canonical: P25891 (Residues: 22-162, 190-288; Coverage: 80%)
Gene name: CRIP3
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ADE 3 x ADE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P32
Unit cell:
a: 115.908Å b: 115.908Å c: 44.898Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.232 0.293
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Survey of the year 2007 commercial optical biosensor literature.
Rich et al. (2008)
3 more

2 mentions without citation

Structure-function study of maize ribosome-inactivating protein: implications for the internal inactivation region and the sole glutamate in the active site.
Mak et al. (2007)
1 more