PDBe 2ppq

X-ray diffraction
2Å resolution

Crystal structure of the homoserine kinase from Agrobacterium tumefaciens

Released:
Entry authors: Zhang R, Xu X, Zheng H, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed:
ATP + L-homoserine = ADP + O-phospho-L-homoserine. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homoserine kinase Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 36.47 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8UHA8 (Residues: 1-322; Coverage: 100%)
Gene names: AGR_C_1416, Atu0775, thrB
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 61.338Å b: 100.619Å c: 134.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.187 0.235
Expression system: Escherichia coli BL21