PDBe 2plc

X-ray diffraction
2Å resolution

PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C FROM LISTERIA MONOCYTOGENES

Released:

Function and Biology Details

Reaction catalysed:
1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.23 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P34024 (Residues: 43-316; Coverage: 93%)
Gene names: lmo0201, pic, plcA
Sequence domains: Phosphatidylinositol-specific phospholipase C, X domain
Structure domains: Phosphatidylinositol (PI) phosphodiesterase

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P3121
Unit cell:
a: 82.5Å b: 82.5Å c: 92.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.198 0.249
Expression system: Escherichia coli BL21(DE3)