PDBe 2pl9

X-ray diffraction
2.6Å resolution

Crystal Structure of CheY-Mg(2+)-BeF(3)(-) in Complex with CheZ(C19) Peptide solved from a P2(1)2(1)2 Crystal

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chemotaxis protein CheY Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 128 amino acids
Theoretical weight: 14.01 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A2D5 (Residues: 2-129; Coverage: 99%)
Gene names: STM1916, cheY
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold
Protein phosphatase CheZ Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 19 amino acids
Theoretical weight: 1.95 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Not provided
UniProt:
  • Canonical: P07800 (Residues: 196-214; Coverage: 9%)
Gene names: STM1915, cheZ

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P21212
Unit cell:
a: 68.058Å b: 162.972Å c: 37.341Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.213 0.274
Expression systems:
  • Escherichia coli
  • Not provided