› 2pkx

X-ray diffraction
2.54Å resolution

E.coli response regulator PhoP receiver domain

Released:
DOI: 10.2210/pdb2pkx/pdb
PDB entry 2pkx coloured by chain, front view.
PDB entry 2pkx coloured by chain, side view.
PDB entry 2pkx coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of the receiver domain of the response regulator PhoP from Escherichia coli in the absence and presence of the phosphoryl analog beryllofluoride.
Bachhawat P, Stock AM
J Bacteriol 189 5987-95 (2007)
PMID: 17545283

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150072 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulatory protein PhoP Chains: A, B
Molecule details ›
Chains: A, B
Length: 121 amino acids
Theoretical weight: 14.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P23836 (Residues: 1-121; Coverage: 54%)
Gene names: JW1116, b1130, phoP
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P65
Unit cell:
a: 102.645Å b: 102.645Å c: 62.641Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.206 0.253
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

Structural and mechanistic determinants of c-di-GMP signalling.
Schirmer et al. (2009)
6 more

11 mentions without citation

Molecular Mechanisms of Two-Component Signal Transduction.
Zschiedrich et al. (2016)
10 more