PDBe 2pk9

X-ray diffraction
2.91Å resolution

Structure of the Pho85-Pho80 CDK-cyclin Complex of the Phosphate-responsive Signal Transduction Pathway

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PHO85 cyclin PHO80 Chains: B, D
Molecule details ›
Chains: B, D
Length: 293 amino acids
Theoretical weight: 33.27 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20052 (Residues: 1-293; Coverage: 100%)
Gene names: AGS3, O2505, PHO80, TUP7, UNB293, VAC5, YOL001W
Sequence domains: Cyclin
Cyclin-dependent protein kinase PHO85 Chains: A, C
Molecule details ›
Chains: A, C
Length: 317 amino acids
Theoretical weight: 36.36 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17157 (Residues: 1-305; Coverage: 100%)
Gene names: P7102.18A, PHO85, SSG3, YPL031C
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID, ESRF BEAMLINE ID29
Spacegroup: P3121
Unit cell:
a: 147.775Å b: 147.775Å c: 211.825Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.283 0.281 0.315
Expression system: Escherichia coli BL21(DE3)