PDBe 2phk

X-ray diffraction
2.6Å resolution

THE CRYSTAL STRUCTURE OF A PHOSPHORYLASE KINASE PEPTIDE SUBSTRATE COMPLEX: KINASE SUBSTRATE RECOGNITION

Released:

Function and Biology Details

Reactions catalysed:
2 ATP + phosphorylase b = 2 ADP + phosphorylase a. 
ATP + a protein = ADP + a phosphoprotein. 
ATP + [tau protein] = ADP + [tau protein] phosphate. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
MC-PEPTIDE Chain: B
Molecule details ›
Chain: B
Length: 7 amino acids
Theoretical weight: 939 Da
Source organism: Unidentified
Expression system: Not provided
Phosphorylase b kinase gamma catalytic chain, skeletal muscle/heart isoform Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 31.98 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00518 (Residues: 15-291; Coverage: 72%)
Gene names: PHKG, PHKG1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE D2AM
Spacegroup: P3221
Unit cell:
a: 65.3Å b: 65.3Å c: 145.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.253 0.236 0.3
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)