PDBe 2pfm

X-ray diffraction
2Å resolution

Crystal Structure of Adenylosuccinate Lyase (PurB) from Bacillus anthracis

Released:
Source organism: Bacillus anthracis
Entry authors: Levdikov VM, Blagova EV, Baumgart M, Moroz OV, Wilkinson AJ, Wilson KS

Function and Biology Details

Reaction catalysed:
(1) N(6)-(1,2-dicarboxyethyl)AMP = fumarate + AMP. (2) (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4- carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D- ribosyl)imidazole-4-carboxamide. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylosuccinate lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 444 amino acids
Theoretical weight: 51.12 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q81ZH6 (Residues: 1-435; Coverage: 100%)
Gene names: GBAA_0290, purB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P6122
Unit cell:
a: 154.381Å b: 154.381Å c: 174.989Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.165 0.163 0.208
Expression system: Escherichia coli BL21