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Solution NMR

THE COMPLEX OF CYTOCHROME F AND PLASTOCYANIN DETERMINED WITH PARAMAGNETIC NMR. BASED ON THE STRUCTURES OF CYTOCHROME F AND PLASTOCYANIN, 10 STRUCTURES

Released:
DOI: 10.2210/pdb2pcf/pdb
PDB entry 2pcf coloured by chain, ensemble of 10 models, front view.
PDB entry 2pcf coloured by chain, ensemble of 10 models, side view.
PDB entry 2pcf coloured by chain, ensemble of 10 models, top view.
Source organisms:
Primary publication:
The structure of the complex of plastocyanin and cytochrome f, determined by paramagnetic NMR and restrained rigid-body molecular dynamics.
Ubbink M, Ejdebäck M, Karlsson BG, Bendall DS
Structure 6 323-35 (1998)
PMID: 9551554

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132378 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Plastocyanin, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 99 amino acids
Theoretical weight: 10.42 KDa
Source organism: Spinacia oleracea
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00289 (Residues: 70-168; Coverage: 59%)
Gene name: PETE
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins
Cytochrome f Chain: B
Molecule details ›
Chain: B
Length: 250 amino acids
Theoretical weight: 27.32 KDa
Source organism: Brassica rapa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P36438 (Residues: 36-285; Coverage: 88%)
Gene name: petA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CU 1 x CU
Ligand HEC 1 x HEC
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: DYNAMICS
Expression system: Escherichia coli BL21

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Citations

31 review citations

Structure and function of cytochrome bc complexes.
Berry et al. (2000)
30 more

8 mentions without citation

New direct dynamic models of protein interactions coupled to photosynthetic electron transport reactions.
Riznichenko et al. (2010)
7 more