PDBe 2p80

Solution NMR

Solution structure of the complex between nitrite reductase and pseudoazurin from A. faecalis

Released:
Source organism: Alcaligenes faecalis
Entry authors: Vlasie MD, Ubbink M

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pseudoazurin Chain: D
Molecule details ›
Chain: D
Length: 123 amino acids
Theoretical weight: 13.38 KDa
Source organism: Alcaligenes faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04377 (Residues: 24-146; Coverage: 100%)
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins
Copper-containing nitrite reductase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 341 amino acids
Theoretical weight: 36.88 KDa
Source organism: Alcaligenes faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38501 (Residues: 40-376; Coverage: 98%)
Gene names: nir, nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry, rigid body molecular dynamics, restrained side-chains energy minimization
Chemical shifts: BMR6043  
Expression system: Escherichia coli BL21(DE3)