PDBe 2p6b

X-ray diffraction
2.3Å resolution

Crystal Structure of Human Calcineurin in Complex with PVIVIT Peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
PVIVIT 14-mer Peptide Chain: E
Molecule details ›
Chain: E
Length: 15 amino acids
Theoretical weight: 1.48 KDa
Calcineurin subunit B type 1 Chains: B, D
Molecule details ›
Chains: B, D
Length: 156 amino acids
Theoretical weight: 17.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63098 (Residues: 15-170; Coverage: 92%)
Gene names: CNA2, CNB, PPP3R1
Sequence domains:
Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform Chains: A, C
Molecule details ›
Chains: A, C
Length: 383 amino acids
Theoretical weight: 43.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q08209 (Residues: 1-380; Coverage: 73%)
Gene names: CALNA, CNA, PPP3CA
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 86.104Å b: 89.155Å c: 157.685Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.254
Expression system: Escherichia coli BL21(DE3)