PDBe 2ovr

X-ray diffraction
2.5Å resolution

Structure of the Skp1-Fbw7-CyclinEdegN complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
F-box/WD repeat-containing protein 7 Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 50.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q969H0 (Residues: 263-707; Coverage: 63%)
Gene names: FBW7, FBX30, FBXW7, SEL10
Sequence domains:
Structure domains:
cyclinE N-terminal degron Chain: C
Molecule details ›
Chain: C
Length: 8 amino acids
Theoretical weight: 951 Da
S-phase kinase-associated protein 1 Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P63208 (Residues: 1-69, 82-163; Coverage: 89%)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Sequence domains:
Structure domains: Potassium Channel Kv1.1; Chain A

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: I4122
Unit cell:
a: 232.558Å b: 232.558Å c: 107.659Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.225 0.251
Expression system: Escherichia coli BL21