› 2oqf

X-ray diffraction
2.3Å resolution

Structure of a synthetic, non-natural analogue of RNase A: [N71K(Ade), D83A]RNase A

Released:
DOI: 10.2210/pdb2oqf/pdb
PDB entry 2oqf coloured by chain, front view.
PDB entry 2oqf coloured by chain, side view.
PDB entry 2oqf coloured by chain, top view.
Source organism: Bos taurus
Entry authors: Boerema DJ, Tereshko VA, Zhang JL, He C, Kent SBH

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 124 amino acids
Theoretical weight: 13.8 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand XX1 4 x XX1

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 37.578Å b: 50.534Å c: 62.863Å
α: 85.26° β: 74.31° γ: 82.7°
R-values:
R R work R free
0.236 0.229 0.291
Expression system: Not provided

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