PDBe 2onk

X-ray diffraction
3.1Å resolution

ABC transporter ModBC in complex with its binding protein ModA

Released:
Source organism: Archaeoglobus fulgidus

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Molybdate/tungstate import ATP-binding protein WtpC Chains: A, B, F, G
Molecule details ›
Chains: A, B, F, G
Length: 240 amino acids
Theoretical weight: 27.07 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O30144 (Residues: 1-240; Coverage: 100%)
Gene names: AF_0092, modC, wtpC
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Molybdate/tungstate transport system permease protein WtpB Chains: C, D, H, I
Molecule details ›
Chains: C, D, H, I
Length: 284 amino acids
Theoretical weight: 30.64 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O30143 (Residues: 1-261; Coverage: 100%)
Gene names: AF_0093, modB, wtpB
Sequence domains: Binding-protein-dependent transport system inner membrane component
Structure domains: MetI-like
Molybdate/tungstate-binding protein WtpA Chains: E, J
Molecule details ›
Chains: E, J
Length: 314 amino acids
Theoretical weight: 35.72 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O30142 (Residues: 31-342; Coverage: 98%)
Gene names: AF_0094, modA, wtpA
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 93.032Å b: 171.203Å c: 158.47Å
α: 90° β: 98.79° γ: 90°
R-values:
R R work R free
0.256 0.256 0.283
Expression system: Escherichia coli