PDBe 2olc

X-ray diffraction
2Å resolution

Crystal structure of 5-methylthioribose kinase in complex with ADP-2Ho

Released:
Source organism: Bacillus subtilis
Primary publication:
ADP-2Ho as a phasing tool for nucleotide-containing proteins.
Acta Crystallogr. D Biol. Crystallogr. 63 493-9 (2007)
PMID: 17372354

Function and Biology Details

Reaction catalysed:
ATP + S-methyl-5-thio-D-ribose = ADP + S-methyl-5-thio-alpha-D-ribose 1-phosphate. 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methylthioribose kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 397 amino acids
Theoretical weight: 45.14 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O31663 (Residues: 1-397; Coverage: 100%)
Gene names: BSU13560, mtnK, ykrT
Sequence domains: Phosphotransferase enzyme family
Structure domains: Phosphorylase Kinase; domain 1

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C, RIGAKU
Spacegroup: P21212
Unit cell:
a: 215.2Å b: 83.6Å c: 51.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.214 0.25
Expression system: Escherichia coli BL21(DE3)