› 2ok5

X-ray diffraction
2.3Å resolution

Human Complement factor B

Released:
DOI: 10.2210/pdb2ok5/pdb
PDB entry 2ok5 coloured by chain, front view.
PDB entry 2ok5 coloured by chain, side view.
PDB entry 2ok5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Factor B structure provides insights into activation of the central protease of the complement system.
Milder FJ, Gomes L, Schouten A, Janssen BJ, Huizinga EG, Romijn RA, Hemrika W, Roos A, Daha MR, Gros P
Nat Struct Mol Biol 14 224-8 (2007)
PMID: 17310251

Function and Biology Details

Reaction catalysed: 
Cleavage of Arg-|-Ser bond in complement component C3 alpha-chain to yield C3a and C3b, and Arg-|- bond in complement component C5 alpha-chain to yield C5a and C5b.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Complement factor B Chain: A
Molecule details ›
Chain: A
Length: 752 amino acids
Theoretical weight: 84.44 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P00751 (Residues: 24-764; Coverage: 100%)
Gene names: BF, BFD, CFB
Sequence domains:
Structure domains: Thrombin, subunit H

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 2 x NAG
Ligand GOL 13 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3121
Unit cell:
a: 104.031Å b: 104.031Å c: 151.116Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.195 0.241
Expression system: Homo sapiens

Quick links

Citations

16 review citations

Complement and its role in innate and adaptive immune responses.
Dunkelberger et al. (2010)
15 more

25 mentions without citation

Complement component C3 - The "Swiss Army Knife" of innate immunity and host defense.
Ricklin et al. (2016)
24 more