PDBe 2oje

X-ray diffraction
3Å resolution

Mycoplasma arthritidis-derived mitogen complexed with class II MHC molecule HLA-DR1/HA complex in the presence of EDTA

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero 16-mer
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Superantigen Chains: D, H
Molecule details ›
Chains: D, H
Length: 214 amino acids
Theoretical weight: 25.42 KDa
Source organism: Mycoplasma arthritidis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q48898 (Residues: 25-238; Coverage: 90%)
Sequence domains: Mycoplasma arthritidis-derived mitogen
Structure domains:
Hemagglutinin Chains: C, G
Molecule details ›
Chains: C, G
Length: 13 amino acids
Theoretical weight: 1.51 KDa
Source organism: Influenza A virus (A/Taiwan/0095/1996(H3N2))
Expression system: Not provided
UniProt:
  • Canonical: Q91LS8 (Residues: 260-272; Coverage: 5%)
Gene name: HA
HLA class II histocompatibility antigen, DR alpha chain Chains: A, E
Molecule details ›
Chains: A, E
Length: 180 amino acids
Theoretical weight: 21.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01903 (Residues: 27-206; Coverage: 79%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DRB1-1 beta chain Chains: B, F
Molecule details ›
Chains: B, F
Length: 190 amino acids
Theoretical weight: 22.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04229 (Residues: 30-219; Coverage: 80%)
Gene name: HLA-DRB1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: I222
Unit cell:
a: 137.735Å b: 179.555Å c: 179.952Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.211 0.261
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided
  • Escherichia coli