2oje Citations

Zinc induces dimerization of the class II major histocompatibility complex molecule that leads to cooperative binding to a superantigen.

J. Biol. Chem. 282 5991-6000 (2007)
Cited: 8 times
EuropePMC logo PMID: 17166841

Abstract

Dimerization of class II major histocompatibility complex (MHC) plays an important role in the MHC biological function. Mycoplasma arthritidis-derived mitogen (MAM) is a superantigen that can activate large fractions of T cells bearing specific T cell receptor Vbeta elements. Here we have used structural, sedimentation, and surface plasmon resonance detection approaches to investigate the molecular interactions between MAM and the class II MHC molecule HLA-DR1 in the context of a hemagglutinin peptide-(306-318) (HA). Our results revealed that zinc ion can efficiently induce the dimerization of the HLA-DR1/HA complex. Because the crystal structure of the MAM/HLA-DR1/hemagglutinin complex in the presence of EDTA is nearly identical to the structure of the complex crystallized in the presence of zinc ion, Zn(2+) is evidently not directly involved in the binding between MAM and HLA-DR1. Sedimentation and surface plasmon resonance studies further revealed that MAM binds the HLA-DR1/HA complex with high affinity in a 1:1 stoichiometry, in the absence of Zn(2+). However, in the presence of Zn(2+), a dimerized MAM/HLA-DR1/HA complex can arise through the Zn(2+)-induced DR1 dimer. In the presence of Zn(2+), cooperative binding of MAM to the DR1 dimer was also observed.

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  2. Functional interaction of CD154 protein with α5β1 integrin is totally independent from its binding to αIIbβ3 integrin and CD40 molecules. El Fakhry Y, Alturaihi H, Yacoub D, Liu L, Guo W, Leveillé C, Jung D, Khzam LB, Merhi Y, Wilkins JA, Li H, Mourad W. J. Biol. Chem. 287 18055-18066 (2012)
  3. Pfit is a structurally novel Crohn's disease-associated superantigen. Liu L, Chen H, Brecher MB, Li Z, Wei B, Nandi B, Zhang J, Ling H, Winslow G, Braun J, Li H. PLoS Pathog. 9 e1003837 (2013)
  4. Crystal structure of the Mycoplasma arthritidis-derived mitogen in apo form reveals a 3D domain-swapped dimer. Liu L, Li Z, Guo Y, VanVranken SJ, Mourad W, Li H. J. Mol. Biol. 399 367-376 (2010)
  5. Impaired synthesis of erythropoietin, glutamine synthetase and metallothionein in the skin of NOD/SCID/gamma(c)(null) and Foxn1 nu/nu mice with misbalanced production of MHC class II complex. Danielyan L, Verleysdonk S, Buadze M, Gleiter CH, Buniatian GH. Neurochem. Res. 35 899-908 (2010)