PDBe 2od8

X-ray diffraction
2.8Å resolution

Structure of a peptide derived from Cdc9 bound to PCNA

Released:

Function and Biology Details

Reaction catalysed:
ATP + (deoxyribonucleotide)(n)-3'-hydroxyl + 5'-phospho- (deoxyribonucleotide)(m) = (deoxyribonucleotide)(n+m) + AMP + diphosphate. 

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proliferating cell nuclear antigen Chain: A
Molecule details ›
Chain: A
Length: 258 amino acids
Theoretical weight: 28.94 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15873 (Residues: 1-258; Coverage: 100%)
Gene names: POL30, YBR0811, YBR088C
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen
DNA ligase 1 Chain: B
Molecule details ›
Chain: B
Length: 22 amino acids
Theoretical weight: 2.46 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
  • Canonical: P04819 (Residues: 32-53; Coverage: 3%)
Gene names: CDC9, YDL164C

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: I23
Unit cell:
a: 139.28Å b: 139.28Å c: 139.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.247 0.285
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided