PDBe 2ocf

X-ray diffraction
2.95Å resolution

Human estrogen receptor alpha ligand-binding domain in complex with estradiol and the E2#23 FN3 monobody

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ugl-Y3 Chain: D
Molecule details ›
Chain: D
Length: 121 amino acids
Theoretical weight: 13.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02751 (Residues: 1447-1540; Coverage: 4%)
Gene names: FN, FN1
Sequence domains: Fibronectin type III domain
Structure domains: Immunoglobulins
Estrogen receptor Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 33.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03372 (Residues: 298-595; Coverage: 50%)
Gene names: ESR, ESR1, NR3A1
Sequence domains:
Structure domains: Retinoid X Receptor

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P6122
Unit cell:
a: 119.219Å b: 119.219Å c: 132.999Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.191 0.251
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)