› 2ocf

X-ray diffraction
2.95Å resolution

Human estrogen receptor alpha ligand-binding domain in complex with estradiol and the E2#23 FN3 monobody

Released:
DOI: 10.2210/pdb2ocf/pdb
PDB entry 2ocf coloured by chain, front view.
PDB entry 2ocf coloured by chain, side view.
PDB entry 2ocf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Probing protein conformational changes in living cells by using designer binding proteins: application to the estrogen receptor.
Koide A, Abbatiello S, Rothgery L, Koide S
Proc Natl Acad Sci U S A 99 1253-8 (2002)
PMID: 11818562

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136143 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Estrogen receptor Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 33.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03372 (Residues: 298-595; Coverage: 50%)
Gene names: ESR, ESR1, NR3A1
Sequence domains:
Structure domains: Retinoid X Receptor
Ugl-Y3 Chain: D
Molecule details ›
Chain: D
Length: 121 amino acids
Theoretical weight: 13.25 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P02751 (Residues: 1538-1631; Coverage: 4%)
Gene names: FN, FN1
Sequence domains: Fibronectin type III domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand EST 1 x EST
1 modified residue:
Ligand CME 2 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P6122
Unit cell:
a: 119.219Å b: 119.219Å c: 132.999Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.191 0.251
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

19 review citations

Engineering novel binding proteins from nonimmunoglobulin domains.
Binz et al. (2005)
18 more

22 mentions without citation

Engineering of recombinant crystallization chaperones.
Koide S. (2009)
21 more