2o9s Citations

Paxillin and ponsin interact in nascent costameres of muscle cells.


Muscle differentiation requires the transition from motile myoblasts to sessile myotubes and the assembly of a highly regular contractile apparatus. This striking cytoskeletal remodelling is coordinated with a transformation of focal adhesion-like cell-matrix contacts into costameres. To assess mechanisms underlying this differentiation process, we searched for muscle specific-binding partners of paxillin. We identified an interaction of paxillin with the vinexin adaptor protein family member ponsin in nascent costameres during muscle differentiation, which is mediated by an interaction of the second src homology domain 3 (SH3) domain of ponsin with the proline-rich region of paxillin. To understand the molecular basis of this interaction, we determined the structure of this SH3 domain at 0.83 A resolution, as well as its complex with the paxillin binding peptide at 1.63 A resolution. Upon binding, the paxillin peptide adopts a polyproline-II helix conformation in the complex. Contrary to the charged SH3 binding interface, the peptide contains only non-polar residues and for the first time such an interaction was observed structurally in SH3 domains. Fluorescence titration confirmed the ponsin/paxillin interaction, characterising it further by a weak binding affinity. Transfection experiments revealed further characteristics of ponsin functions in muscle cells: All three SH3 domains in the C terminus of ponsin appeared to synergise in targeting the protein to force-transducing structures. The overexpression of ponsin resulted in altered muscle cell-matrix contact morphology, suggesting its involvement in the establishment of mature costameres. Further evidence for the role of ponsin in the maintenance of mature mechanotransduction sites in cardiomyocytes comes from the observation that ponsin expression was down-regulated in end-stage failing hearts, and that this effect was reverted upon mechanical unloading. These results provide new insights in how low affinity protein-protein interactions may contribute to a fine tuning of cytoskeletal remodelling processes during muscle differentiation and in adult cardiomyocytes.

Articles - 2o9s mentioned but not cited (4)

  1. Development of a new physics-based internal coordinate mechanics force field and its application to protein loop modeling. Arnautova YA, Abagyan RA, Totrov M. Proteins 79 477-498 (2011)
  2. Compact Representation of Continuous Energy Surfaces for More Efficient Protein Design. Hallen MA, Gainza P, Donald BR. J Chem Theory Comput 11 2292-2306 (2015)
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Reviews citing this publication (3)

  1. The interactome of LIM domain proteins: the contributions of LIM domain proteins to heart failure and heart development. Li A, Ponten F, dos Remedios CG. Proteomics 12 203-225 (2012)
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Articles citing this publication (12)

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  9. Structural basis for recognition of the third SH3 domain of full-length R85 (R85FL)/ponsin by ataxin-7. Jiang YJ, Zhou CJ, Zhou ZR, Wu M, Hu HY. FEBS Lett. 587 2905-2911 (2013)
  10. Repression of the Central Splicing Regulator RBFox2 Is Functionally Linked to Pressure Overload-Induced Heart Failure. Wei C, Qiu J, Zhou Y, Xue Y, Hu J, Ouyang K, Banerjee I, Zhang C, Chen B, Li H, Chen J, Song LS, Fu XD. Cell Rep (2015)
  11. Structural investigation of the interaction between the tandem SH3 domains of c-Cbl-associated protein and vinculin. Zhao D, Wang X, Peng J, Wang C, Li F, Sun Q, Zhang Y, Zhang J, Cai G, Zuo X, Wu J, Shi Y, Zhang Z, Gong Q. J. Struct. Biol. 187 194-205 (2014)
  12. Structural basis of focal adhesion targeting domain-mediated signaling in cardiac hypertrophy. Mohanty P, Bhatnagar S. J. Recept. Signal Transduct. Res. 37 38-50 (2017)