PDBe 2o5g

X-ray diffraction
1.08Å resolution

Calmodulin-smooth muscle light chain kinase peptide complex

Released:
Source organism: Gallus gallus
Entry authors: Valentine KG, Ng HL, Schneeweis JK, Kranz JK, Frederick KK, Alber T, Wand AJ

Function and Biology Details

Reaction catalysed:
ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.72 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62149 (Residues: 2-149; Coverage: 99%)
Gene names: CALM, CAM, RCJMB04_24e7
Sequence domains: EF-hand domain pair
Myosin light chain kinase, smooth muscle, deglutamylated form Chain: B
Molecule details ›
Chain: B
Length: 21 amino acids
Theoretical weight: 2.22 KDa
Source organism: Gallus gallus
Expression system: Not provided
UniProt:
  • Canonical: P11799 (Residues: 1730-1748; Coverage: 1%)
Gene name: Mylk

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 28.861Å b: 56.894Å c: 44.76Å
α: 90° β: 97.45° γ: 90°
R-values:
R R work R free
0.144 0.142 0.165
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided