PDBe 2nzy

X-ray diffraction
2.05Å resolution

Crystal Structure of alpha1,3-Fucosyltransferase with GDP-fucose


Function and Biology Details

Reaction catalysed:
GDP-beta-L-fucose + (1->4)-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R = GDP + (1->4)-beta-D-galactosyl-(alpha-(1->3)-L-fucosyl)-N-acetyl-D-glucosaminyl-R
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Alpha-(1,3)-fucosyltransferase FucT Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 371 amino acids
Theoretical weight: 43.12 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli BL21(DE3)
  • Canonical: O30511 (Residues: 1-363; Coverage: 76%)
Gene name: fucT
Sequence domains: Glycosyltransferase family 10 (fucosyltransferase) C-term
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21212
Unit cell:
a: 103.962Å b: 135.74Å c: 96.19Å
α: 90° β: 90° γ: 90°
R R work R free
0.192 0.19 0.246
Expression system: Escherichia coli BL21(DE3)