PDBe 2nzw

X-ray diffraction
1.9Å resolution

Crystal Structure of alpha1,3-Fucosyltransferase

Released:

Function and Biology Details

Reaction catalysed:
GDP-beta-L-fucose + (1->4)-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R = GDP + (1->4)-beta-D-galactosyl-(alpha-(1->3)-L-fucosyl)-N-acetyl-D- glucosaminyl-R. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-(1,3)-fucosyltransferase FucT Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 371 amino acids
Theoretical weight: 43.12 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O30511 (Residues: 1-363; Coverage: 76%)
Gene name: fucT
Sequence domains: Glycosyltransferase family 10 (fucosyltransferase) C-term

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL12B2
Spacegroup: P21212
Unit cell:
a: 104.545Å b: 136.196Å c: 96.829Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.242
Expression system: Escherichia coli BL21(DE3)