PDBe 2nzf

X-ray diffraction
2.28Å resolution

Structure of beta-lactamase II from Bacillus cereus. R121H, C221S double mutant. Space group C2.


Function and Biology Details

Reaction catalysed:
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Metallo-beta-lactamase type 2 Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 24.26 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P04190 (Residues: 37-257; Coverage: 97%)
Gene name: blm
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: C2
Unit cell:
a: 52.587Å b: 61.156Å c: 68.634Å
α: 90° β: 93.11° γ: 90°
R R work R free
0.167 0.163 0.238
Expression system: Escherichia coli BL21(DE3)