PDBe 2nxx

X-ray diffraction
2.75Å resolution

Crystal Structure of the Ligand-Binding Domains of the T.castaneum (Coleoptera) Heterodimer EcrUSP Bound to Ponasterone A

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ultraspiracle nuclear receptor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 235 amino acids
Theoretical weight: 26.58 KDa
Source organism: Tribolium castaneum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A1JUG2 (Residues: 174-407; Coverage: 58%)
Gene name: usp
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Ecdysone receptor (Isoform A) Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 248 amino acids
Theoretical weight: 28.88 KDa
Source organism: Tribolium castaneum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A1JUG3 (Residues: 306-549; Coverage: 44%)
Gene name: ecr-a
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 77.54Å b: 89.628Å c: 160.247Å
α: 90° β: 91.97° γ: 90°
R-values:
R R work R free
0.249 0.249 0.281
Expression system: Escherichia coli BL21(DE3)