PDBe 2nug

X-ray diffraction
1.7Å resolution

Crystal structure of RNase III from Aquifex aeolicus complexed with ds-RNA at 1.7-Angstrom Resolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Ribonuclease 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 26.15 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67082 (Residues: 1-221; Coverage: 100%)
Gene names: aq_946, rnc
Sequence domains:
Structure domains:
5'-R(P*AP*AP*GP*GP*UP*CP*AP*UP*UP*CP*G)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 11 nucleotides
Theoretical weight: 3.51 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-R(P*AP*GP*UP*GP*GP*CP*CP*UP*UP*GP*C)-3' Chains: E, F
Molecule details ›
Chains: E, F
Length: 11 nucleotides
Theoretical weight: 3.5 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21212
Unit cell:
a: 95.4Å b: 119.9Å c: 58.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.22
Expression systems:
  • Escherichia coli
  • Not provided