PDBe 2nue

X-ray diffraction
2.9Å resolution

Crystal structure of RNase III from Aquifex aeolicus complexed with ds-RNA at 2.9-Angstrom Resolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Ribonuclease 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 26.15 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67082 (Residues: 1-221; Coverage: 100%)
Gene names: aq_946, rnc
Sequence domains:
Structure domains:
46-MER Chain: C
Molecule details ›
Chain: C
Length: 46 nucleotides
Theoretical weight: 14.82 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 101.596Å b: 101.657Å c: 67.082Å
α: 90° β: 103.69° γ: 90°
R-values:
R R work R free
0.238 0.238 0.275
Expression systems:
  • Escherichia coli
  • Not provided