› 2nu4

X-ray diffraction
1.75Å resolution

Accommodation of positively-charged residues in a hydrophobic specificity pocket: Crystal structures of SGPB in complex with OMTKY3 variants Lys18I and Arg18I

Released:
DOI: 10.2210/pdb2nu4/pdb
PDB entry 2nu4 coloured by chain, front view.
PDB entry 2nu4 coloured by chain, side view.
PDB entry 2nu4 coloured by chain, top view.
Source organisms:
Entry authors: Bateman KS, Anderson S, Lu W, Qasim MA, Laskowski Jr M, James MNG

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins with trypsin-like specificity.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133489 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Streptogrisin-B Chain: E
Molecule details ›
Chain: E
Length: 185 amino acids
Theoretical weight: 18.65 KDa
Source organism: Streptomyces griseus
UniProt:
  • Canonical: P00777 (Residues: 115-299; Coverage: 71%)
Gene name: sprB
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Ovomucoid Chain: I
Molecule details ›
Chain: I
Length: 51 amino acids
Theoretical weight: 5.6 KDa
Source organism: Meleagris gallopavo
Expression system: Escherichia coli
UniProt:
  • Canonical: P68390 (Residues: 135-185; Coverage: 28%)
Sequence domains: Kazal-type serine protease inhibitor domain
Structure domains: Wheat Germ Agglutinin (Isolectin 2); domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 45.452Å b: 54.689Å c: 45.633Å
α: 90° β: 119.33° γ: 90°
R-values:
R R work R free
0.17 0.17 not available
Expression system: Escherichia coli

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