PDBe 2ntb

X-ray diffraction
1.8Å resolution

Crystal structure of pectin methylesterase in complex with hexasaccharide V

Released:

Function and Biology Details

Reaction catalysed:
Pectin + n H(2)O = n methanol + pectate. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pectinesterase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 342 amino acids
Theoretical weight: 36.99 KDa
Source organism: Dickeya dadantii 3937
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C1A9 (Residues: 25-366; Coverage: 100%)
Gene names: Dda3937_03374, pem, pemA
Sequence domains: Pectinesterase
Structure domains: Single-stranded right-handed beta-helix, Pectin lyase-like

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 51.094Å b: 85.33Å c: 97.803Å
α: 90° β: 93.55° γ: 90°
R-values:
R R work R free
0.207 0.205 0.244
Expression system: Escherichia coli