› 2n71

Solution NMR

NMR structure of CmPI-II, a serin protease inhibitor isolated from mollusk Cenchitis muricatus

Released:
DOI: 10.2210/pdb2n71/pdb
PDB entry 2n71 coloured by chain, ensemble of 15 models, front view.
PDB entry 2n71 coloured by chain, ensemble of 15 models, side view.
PDB entry 2n71 coloured by chain, ensemble of 15 models, top view.
Source organism: Cenchritis muricatus
Primary publication:
NMR structure of CmPI-II, a non-classical Kazal protease inhibitor: Understanding its conformational dynamics and subtilisin A inhibition.
Cabrera-Muñoz A, Valiente PA, Rojas L, Alonso-Del-Rivero Antigua M, Pires JR
J Struct Biol 206 280-294 (2019)
PMID: 30930219

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160907 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protease inhibitor 2 Chain: A
Molecule details ›
Chain: A
Length: 50 amino acids
Theoretical weight: 5.49 KDa
Source organism: Cenchritis muricatus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P84755 (Residues: 1-50; Coverage: 100%)
Sequence domains: Kazal-type serine protease inhibitor domain
Structure domains: Wheat Germ Agglutinin (Isolectin 2); domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 85%
Refinement method: simulated annealing, torsion angle dynamics
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

Cysteines and Disulfide Bonds as Structure-Forming Units: Insights From Different Domains of Life and the Potential for Characterization by NMR.
Wiedemann et al. (2020)
1 more