› 2m3v

Solution NMR

Solution structure of tyrosine phosphatase related to biofilm formation A (TpbA) from Pseudomonas aeruginosa

Released:
DOI: 10.2210/pdb2m3v/pdb
PDB entry 2m3v coloured by chain, ensemble of 20 models, front view.
PDB entry 2m3v coloured by chain, ensemble of 20 models, side view.
PDB entry 2m3v coloured by chain, ensemble of 20 models, top view.
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Primary publication:
Ligand binding reduces conformational flexibility in the active site of tyrosine phosphatase related to biofilm formation A (TpbA) from Pseudomonasaeruginosa.
Koveal D, Clarkson MW, Wood TK, Page R, Peti W
J Mol Biol 425 2219-31 (2013)
PMID: 23524133

Function and Biology Details

Reactions catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101707 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual specificity protein phosphatase TpbA Chain: A
Molecule details ›
Chain: A
Length: 193 amino acids
Theoretical weight: 21.37 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H2ZFK2 (Residues: 29-218; Coverage: 100%)
Gene names: PA14_13660, tpbA
Sequence domains: Tyrosine phosphatase family
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 76%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Protein Phosphatases of Pathogenic Bacteria: Role in Physiology and Virulence.
Sajid et al. (2015)
3 more

1 mention without citation

Structural and Biochemical Analysis of Tyrosine Phosphatase Related to Biofilm Formation A (TpbA) from the Opportunistic Pathogen Pseudomonas aeruginosa PAO1.
Xu et al. (2015)