PDBe 2lpz

Solid-state NMR

Atomic model of the Type-III Secretion System Needle

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo 29-mer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein PrgI Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z, a, b, c
Length: 80 amino acids
Theoretical weight: 8.86 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P41784 (Residues: 1-80; Coverage: 100%)
Gene names: STM2873, prgI
Sequence domains: Type III secretion needle MxiH, YscF, SsaG, EprI, PscF, EscF

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 2%
Refinement method: Monte Carlo and gradient-based optimization
Expression system: Escherichia coli BL21(DE3)