PDBe 2len

Solution NMR

Solution structure of UCHL1 S18Y variant

Released:
Source organism: Homo sapiens
Entry author: Tse H

Function and Biology Details

Reaction catalysed:
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). 

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin carboxyl-terminal hydrolase isozyme L1 Chain: A
Molecule details ›
Chain: A
Length: 231 amino acids
Theoretical weight: 26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09936 (Residues: 1-223; Coverage: 100%)
Gene name: UCHL1
Structure domains: Ubiquitin C-terminal Hydrolase Uch-l3

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 78%
Refinement method: molecular dynamics
Chemical shifts: BMR17260  
Expression system: Escherichia coli