PDBe 2le0

Solution NMR

PARP BRCT Domain

Released:
Source organism: Rattus norvegicus
Primary publication:
Structural studies of the PARP-1 BRCT domain.
OpenAccess logo BMC Struct. Biol. 11 37 (2011)
PMID: 21967661

Function and Biology Details

Reaction catalysed:
NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D- ribosyl)(n+1)-acceptor. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly [ADP-ribose] polymerase 1 Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 11.67 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P27008 (Residues: 389-487; Coverage: 10%)
Gene names: Adprt, Parp1
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 81%
Refinement method: simulated annealing, simulated annealing
Chemical shifts: BMR17687  
Expression system: Escherichia coli