› 2lcs

Solution NMR

Yeast Nbp2p SH3 domain in complex with a peptide from Ste20p

Released:
DOI: 10.2210/pdb2lcs/pdb
PDB entry 2lcs coloured by chain, ensemble of 20 models, front view.
PDB entry 2lcs coloured by chain, ensemble of 20 models, side view.
PDB entry 2lcs coloured by chain, ensemble of 20 models, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Distinct peptide binding specificities of Src homology 3 (SH3) protein domains can be determined by modulation of local energetics across the binding interface.
Gorelik M, Davidson AR
J Biol Chem 287 9168-77 (2012)
PMID: 22277653

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169900 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NAP1-binding protein 2 Chain: A
Molecule details ›
Chain: A
Length: 73 amino acids
Theoretical weight: 8.39 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12163 (Residues: 110-172; Coverage: 27%)
Gene names: NBP2, YDR162C
Sequence domains: SH3 domain
Structure domains: SH3 Domains
Serine/threonine-protein kinase STE20 Chain: B
Molecule details ›
Chain: B
Length: 16 amino acids
Theoretical weight: 1.64 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
  • Canonical: Q03497 (Residues: 468-483; Coverage: 2%)
Gene names: STE20, YHL007C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 86%
Refinement method: torsion angle dynamics
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Interactions by Disorder - A Matter of Context.
Bugge et al. (2020)
10 other articles

2 mentions without citation

Distinct peptide binding specificities of Src homology 3 (SH3) protein domains can be determined by modulation of local energetics across the binding interface.
Gorelik et al. (2012)
1 more