› 2kf3

Solution NMR

Barnase, low pressure reference NMR structure

Released:
DOI: 10.2210/pdb2kf3/pdb
PDB entry 2kf3 coloured by chain, ensemble of 10 models, front view.
PDB entry 2kf3 coloured by chain, ensemble of 10 models, side view.
PDB entry 2kf3 coloured by chain, ensemble of 10 models, top view.
Source organism: Bacillus amyloliquefaciens
Primary publication:
Pressure-dependent structure changes in barnase on ligand binding reveal intermediate rate fluctuations.
Wilton DJ, Kitahara R, Akasaka K, Pandya MJ, Williamson MP
Biophys J 97 1482-90 (2009)
PMID: 19720037

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132802 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease Chain: A
Molecule details ›
Chain: A
Length: 110 amino acids
Theoretical weight: 12.33 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00648 (Residues: 48-157; Coverage: 89%)
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: molecular dynamics
Expression system: Escherichia coli

Quick links

Citations

2 review citations

High-pressure macromolecular crystallography and NMR: status, achievements and prospects.
Fourme et al. (2012)
1 more

2 mentions without citation

Automated real-space refinement of protein structures using a realistic backbone move set.
Haddadian et al. (2011)
1 more