› 2kej

Solution NMR

Solution structure of a dimer of LAC repressor DNA-binding domain complexed to its natural operator O2

Released:
DOI: 10.2210/pdb2kej/pdb
PDB entry 2kej coloured by chain, ensemble of 20 models, front view.
PDB entry 2kej coloured by chain, ensemble of 20 models, side view.
PDB entry 2kej coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia coli K-12
Primary publication:
Specificity and affinity of Lac repressor for the auxiliary operators O2 and O3 are explained by the structures of their protein-DNA complexes.
Romanuka J, Folkers GE, Biris N, Tishchenko E, Wienk H, Bonvin AM, Kaptein R, Boelens R
J Mol Biol 390 478-89 (2009)
PMID: 19450607

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115471 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Lactose operon repressor Chains: A, B
Molecule details ›
Chains: A, B
Length: 62 amino acids
Theoretical weight: 6.82 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P03023 (Residues: 1-62; Coverage: 17%)
Gene names: JW0336, b0345, lacI
Sequence domains: Bacterial regulatory proteins, lacI family
Structure domains: lambda repressor-like DNA-binding domains
DNA (5'-D(*GP*AP*AP*AP*TP*GP*TP*GP*AP*GP*CP*GP*AP*GP*TP*AP*AP*CP*AP*AP*CP*CP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 23 nucleotides
Theoretical weight: 7.15 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
DNA (5'-D(P*CP*GP*GP*TP*TP*GP*TP*TP*AP*CP*TP*CP*GP*CP*TP*CP*AP*CP*AP*TP*TP*TP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 23 nucleotides
Theoretical weight: 6.97 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

3 review citations

Making the bend: DNA tertiary structure and protein-DNA interactions.
Harteis et al. (2014)
2 more

2 mentions without citation

Alignment of major-groove hydrogen bond arrays uncovers shared information between different DNA sequences that bind the same protein.
Sedhom et al. (2022)
1 more