PDBe 2k7a

Solution NMR

Ensemble Structures of the binary complex between the SH3 and SH2 domain of interleukin-2 tyrosine kinase.

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase ITK/TSK Chain: B
Molecule details ›
Chain: B
Length: 110 amino acids
Theoretical weight: 12.68 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03526 (Residues: 238-344; Coverage: 17%)
Gene names: Emt, Itk, Tlk, Tsk
Sequence domains: SH2 domain
Structure domains: SHC Adaptor Protein
Tyrosine-protein kinase ITK/TSK Chain: A
Molecule details ›
Chain: A
Length: 63 amino acids
Theoretical weight: 7.36 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03526 (Residues: 177-237; Coverage: 10%)
Gene names: Emt, Itk, Tlk, Tsk
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 81%
Refinement method: simulated annealing
Chemical shifts: BMR16809  
Expression system: Escherichia coli