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NMR Solution structure of the C-terminal domain (T94-Y172) of the human centrin 2 in complex with a repeat sequence of human Sfi1 (R641-T660)

Solution NMR
PDB entry 2k2i coloured by chain, ensemble of 15 models, front view.
PDB entry 2k2i coloured by chain, ensemble of 15 models, side view.
PDB entry 2k2i coloured by chain, ensemble of 15 models, top view.
Source organism: Homo sapiens
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Centrin-2 and Protein SFI1 homolog
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Multimeric state: hetero dimer
Accessible surface area: 7163.9 Å2
Buried surface area: 1242.35 Å2
Dissociation area: 621.17 Å2
Dissociation energy (ΔGdiss): 7.17 kcal/mol
Dissociation entropy (TΔSdiss): 8.8 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-108725

Macromolecules

Centrin-2
Chain: A
Length: 79 amino acids
Theoretical weight: 9.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P41208 (Residues: 94-172; Coverage: 46%)
Gene names: CALT, CEN2, CETN2
Pfam: EF-hand domain pair
InterPro:
Centrin-2 in PDB entry 2k2i, assembly 1, front view.
Centrin-2 in PDB entry 2k2i, assembly 1, side view.
Centrin-2 in PDB entry 2k2i, assembly 1, top view.
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Protein SFI1 homolog
Chain: B
Length: 20 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: A8K8P3 (Residues: 641-660; Coverage: 2%)
Gene names: KIAA0542, SFI1
Protein SFI1 homolog in PDB entry 2k2i, assembly 1, front view.
Protein SFI1 homolog in PDB entry 2k2i, assembly 1, side view.
Protein SFI1 homolog in PDB entry 2k2i, assembly 1, top view.
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