PDBe 2ju0

Solution NMR

Structure of Yeast Frequenin bound to PdtIns 4-kinase

Released:

Function and Biology Details

Reaction catalysed:
ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo- inositol 4-phosphate. 
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calcium-binding protein NCS-1 Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 22.04 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q06389 (Residues: 1-190; Coverage: 100%)
Gene names: D9481.6, FRQ1, NCS1, YDR373W
Sequence domains: EF hand
Structure domains: EF-hand
Phosphatidylinositol 4-kinase PIK1 Chain: B
Molecule details ›
Chain: B
Length: 52 amino acids
Theoretical weight: 5.63 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P39104 (Residues: 121-172; Coverage: 5%)
Gene names: N0795, PIK1, YNL267W
Sequence domains: Yeast phosphatidylinositol-4-OH kinase Pik1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)